Coding

Part:BBa_K863015:Design

Designed by: Isabel Huber   Group: iGEM12_Bielefeld-Germany   (2012-09-20)

xccl laccase from Xanthomonas campestris with T7 promoter, RBS and His-tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 196
    Illegal PstI site found at 1582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 196
    Illegal PstI site found at 1582
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1131
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 196
    Illegal PstI site found at 1582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 196
    Illegal PstI site found at 1582
    Illegal NgoMIV site found at 267
    Illegal NgoMIV site found at 372
    Illegal NgoMIV site found at 623
    Illegal NgoMIV site found at 816
    Illegal NgoMIV site found at 820
    Illegal NgoMIV site found at 1206
    Illegal NgoMIV site found at 1227
    Illegal AgeI site found at 554
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers for isolation of the gene out of genomic DNA with BioBrick Prefix, pT7 promoter and RBS in the fwd primer and Suffix and His-tag in the rev primer.
fwd: 5'-ACGTGAATTCGCGGCCGCTTCTAGAGtaatacgactcactatagggagagaggagaaaaATGTCATTCGATCCCTTGTC-3'
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTATTATTAGTGATGGTGATGGTGATGTGCCTCCACCCGCACTT-3'

Source

The gene sequence was isolated from Xanthomonas campestris pv. campestris B100.

References

Hsiao, Y. M., Y. F. Liu, et al. (2011). "Functional characterization of copA gene encoding multicopper oxidase in Xanthomonas campestris pv. campestris." J Agric Food Chem 59(17): 9290-9302.